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modified pbluescript ii ks (+) vector  (Agilent technologies)


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    Agilent technologies modified pbluescript ii ks (+) vector
    Modified Pbluescript Ii Ks (+) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/modified pbluescript ii ks (+) vector/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    modified pbluescript ii ks (+) vector - by Bioz Stars, 2026-03
    90/100 stars

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    Construction scheme for the cloning plasmid pBluescript I with restriction sites and fragments of the factor VIII gene: gene fragment from vector IOH10704, gene fragments A–C; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of blood coagulation factor VIII (Vector NTI AdvanceTM10)

    Journal: BioTechnologia

    Article Title: Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system

    doi: 10.5114/bta.2023.130728

    Figure Lengend Snippet: Construction scheme for the cloning plasmid pBluescript I with restriction sites and fragments of the factor VIII gene: gene fragment from vector IOH10704, gene fragments A–C; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of blood coagulation factor VIII (Vector NTI AdvanceTM10)

    Article Snippet: The plasmid vector pBluescript II (GenBank Accession No. ATCC 87047) carries the ColE1 replicon and the ampicillin resistance gene (ampR).

    Techniques: Cloning, Plasmid Preparation, Mutagenesis, Sequencing, Coagulation

    Primers for the construction of the cloning plasmid  pBluescript  I

    Journal: BioTechnologia

    Article Title: Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system

    doi: 10.5114/bta.2023.130728

    Figure Lengend Snippet: Primers for the construction of the cloning plasmid pBluescript I

    Article Snippet: The plasmid vector pBluescript II (GenBank Accession No. ATCC 87047) carries the ColE1 replicon and the ampicillin resistance gene (ampR).

    Techniques: Cloning, Plasmid Preparation

    Construction scheme for the cloning plasmid pBluescript + Fragment 1 + Fragment 2 + Fragment 3; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of the blood coagulation factor VIII; AmpR – ampicillin resistance gene, ori – origin of replication (Vector NTI AdvanceTM10)

    Journal: BioTechnologia

    Article Title: Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system

    doi: 10.5114/bta.2023.130728

    Figure Lengend Snippet: Construction scheme for the cloning plasmid pBluescript + Fragment 1 + Fragment 2 + Fragment 3; site-directed mutagenesis was necessary in order to change restriction sites into the original sequence of the blood coagulation factor VIII; AmpR – ampicillin resistance gene, ori – origin of replication (Vector NTI AdvanceTM10)

    Article Snippet: The plasmid vector pBluescript II (GenBank Accession No. ATCC 87047) carries the ColE1 replicon and the ampicillin resistance gene (ampR).

    Techniques: Cloning, Plasmid Preparation, Mutagenesis, Sequencing, Coagulation