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modified pbluescript ii ks (+) vector  (Agilent technologies)


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    Structured Review

    Agilent technologies modified pbluescript ii ks (+) vector
    Modified Pbluescript Ii Ks (+) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+ks++vector/bio_rxiv__2024__05__24__595694-255-41-46
    Average 90 stars, based on 1 article reviews
    modified pbluescript ii ks (+) vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Tetrahydropyrazolopyrimidine compounds
    Article Snippet: TLR4 transfected cells were also engineered to express human MD-2 co-receptor [MD-2 cDNA (Accession No. NM_015364) was cloned into the pEF-BOS vector] and were supplemented with 10 nM soluble CD14 (R&D Systems) in the media to optimize LPS responsiveness. .. Human TLR9 cDNA (Accession No. NM_017442) was cloned into the pBluescript II KS vector (Agilent). .. Human TLR7 cDNA (Accession No. NM_016562) was obtained from OriGene.

    Article Title: Selectively substituted quinoline compounds
    Article Snippet: TLR4 transfected cells were also engineered to express human MD-2 co-receptor [MD-2 cDNA (Accession No. NM_015364) was cloned into the pEF-BOS vector] and were supplemented with 10 nM soluble CD14 (R&D Systems) in the media to optimize LPS responsiveness. .. Human TLR9 cDNA (Accession No. NM_017442) was cloned into the pBluescript II KS vector (Agilent). .. Human TLR7 cDNA (Accession No. NM_016562) was obtained from OriGene.

    Article Title: Regulatory effects of introduction of an exogenous FGF2 gene on other growth factor genes in a healing tendon.
    Article Snippet: .. A segment containing FGF2 gene preserved in a plasmid was cut was cloned to the pBluescript II KS vector (Stratagene, La Jolla, CA) at EcoRI and XhoI restriction sites to lengthen the segment. .. Subsequently, a 2.7-kb fragment containing the FGF2 gene sequences was cut from the pBluescript 11 KS vector and was inserted to pAAV2-MCS vector (Stratagene) to produce pAAV2-FGF2, which harbors a cytomegalovirus immediate early promoter.

    Article Title: Tetrahydropyrazolopyrimidine compounds
    Article Snippet: TLR4 transfected cells were also engineered to express human MD-2 co-receptor [MD-2 cDNA (Accession No. NM—015364) was cloned into the pEF-BOS vector] and were supplemented with 10 nM soluble CD14 (R&D Systems) in the media to optimize LPS responsiveness. .. Human TLR9 cDNA (Accession No. NM—017442) was cloned into the pBluescript II KS vector (Agilent). .. Human TLR7 cDNA (Accession No. NM—016562) was obtained from OriGene.

    Article Title: Hypoallergenic hybrid proteins of major group 1 and 2 mite allergens for use in the treatment of allergies
    Article Snippet: .. The PCR product obtained was isolated, cloned in the pBluescript II KS vector (Stratagene) and sequenced. ..

    Plasmid Preparation:

    Article Title: Tetrahydropyrazolopyrimidine compounds
    Article Snippet: TLR4 transfected cells were also engineered to express human MD-2 co-receptor [MD-2 cDNA (Accession No. NM_015364) was cloned into the pEF-BOS vector] and were supplemented with 10 nM soluble CD14 (R&D Systems) in the media to optimize LPS responsiveness. .. Human TLR9 cDNA (Accession No. NM_017442) was cloned into the pBluescript II KS vector (Agilent). .. Human TLR7 cDNA (Accession No. NM_016562) was obtained from OriGene.

    Article Title: Selectively substituted quinoline compounds
    Article Snippet: TLR4 transfected cells were also engineered to express human MD-2 co-receptor [MD-2 cDNA (Accession No. NM_015364) was cloned into the pEF-BOS vector] and were supplemented with 10 nM soluble CD14 (R&D Systems) in the media to optimize LPS responsiveness. .. Human TLR9 cDNA (Accession No. NM_017442) was cloned into the pBluescript II KS vector (Agilent). .. Human TLR7 cDNA (Accession No. NM_016562) was obtained from OriGene.

    Article Title: Regulatory effects of introduction of an exogenous FGF2 gene on other growth factor genes in a healing tendon.
    Article Snippet: .. A segment containing FGF2 gene preserved in a plasmid was cut was cloned to the pBluescript II KS vector (Stratagene, La Jolla, CA) at EcoRI and XhoI restriction sites to lengthen the segment. .. Subsequently, a 2.7-kb fragment containing the FGF2 gene sequences was cut from the pBluescript 11 KS vector and was inserted to pAAV2-MCS vector (Stratagene) to produce pAAV2-FGF2, which harbors a cytomegalovirus immediate early promoter.

    Article Title: Preparation and uses of gene sequences encoding chimerical glycosyltransferases with optimized glycosylation activity
    Article Snippet: .. The fragment was then HindIII/BamHI digested and inserted into the pFLAg-CMV1 mammalian expression vector and subcloned into the pBluescript II KS vector (Stratagene) for further constructions. ..

    Article Title: Tetrahydropyrazolopyrimidine compounds
    Article Snippet: TLR4 transfected cells were also engineered to express human MD-2 co-receptor [MD-2 cDNA (Accession No. NM—015364) was cloned into the pEF-BOS vector] and were supplemented with 10 nM soluble CD14 (R&D Systems) in the media to optimize LPS responsiveness. .. Human TLR9 cDNA (Accession No. NM—017442) was cloned into the pBluescript II KS vector (Agilent). .. Human TLR7 cDNA (Accession No. NM—016562) was obtained from OriGene.

    Article Title: Hypoallergenic hybrid proteins of major group 1 and 2 mite allergens for use in the treatment of allergies
    Article Snippet: .. The PCR product obtained was isolated, cloned in the pBluescript II KS vector (Stratagene) and sequenced. ..

    Article Title: Successive duplication-divergence mechanisms at the RCO locus contributed to leaf shape diversity in the Brassicaceae.
    Article Snippet: .. Molecular cloning and plant transformation Genomic fragments and reporter constructs were introduced into pBluescript II KS vector (Stratagene, pBlueMLAPUCAP) using Seamless Ligation Cloning Extract (SLICE) (Zhang et al., 2012). ..

    Expressing:

    Article Title: Preparation and uses of gene sequences encoding chimerical glycosyltransferases with optimized glycosylation activity
    Article Snippet: .. The fragment was then HindIII/BamHI digested and inserted into the pFLAg-CMV1 mammalian expression vector and subcloned into the pBluescript II KS vector (Stratagene) for further constructions. ..

    Polymerase Chain Reaction:

    Article Title: Hypoallergenic hybrid proteins of major group 1 and 2 mite allergens for use in the treatment of allergies
    Article Snippet: .. The PCR product obtained was isolated, cloned in the pBluescript II KS vector (Stratagene) and sequenced. ..

    Isolation:

    Article Title: Hypoallergenic hybrid proteins of major group 1 and 2 mite allergens for use in the treatment of allergies
    Article Snippet: .. The PCR product obtained was isolated, cloned in the pBluescript II KS vector (Stratagene) and sequenced. ..

    other:

    Article Title: Selectively substituted quinoline compounds
    Article Snippet: Human TLR4 cDNA (Accession No. NM_138554) was cloned into pcDNA 3.0 expression vector (Invitrogen).

    Molecular Cloning:

    Article Title: Successive duplication-divergence mechanisms at the RCO locus contributed to leaf shape diversity in the Brassicaceae.
    Article Snippet: .. Molecular cloning and plant transformation Genomic fragments and reporter constructs were introduced into pBluescript II KS vector (Stratagene, pBlueMLAPUCAP) using Seamless Ligation Cloning Extract (SLICE) (Zhang et al., 2012). ..

    Transformation Assay:

    Article Title: Successive duplication-divergence mechanisms at the RCO locus contributed to leaf shape diversity in the Brassicaceae.
    Article Snippet: .. Molecular cloning and plant transformation Genomic fragments and reporter constructs were introduced into pBluescript II KS vector (Stratagene, pBlueMLAPUCAP) using Seamless Ligation Cloning Extract (SLICE) (Zhang et al., 2012). ..

    Construct:

    Article Title: Successive duplication-divergence mechanisms at the RCO locus contributed to leaf shape diversity in the Brassicaceae.
    Article Snippet: .. Molecular cloning and plant transformation Genomic fragments and reporter constructs were introduced into pBluescript II KS vector (Stratagene, pBlueMLAPUCAP) using Seamless Ligation Cloning Extract (SLICE) (Zhang et al., 2012). ..

    Ligation:

    Article Title: Successive duplication-divergence mechanisms at the RCO locus contributed to leaf shape diversity in the Brassicaceae.
    Article Snippet: .. Molecular cloning and plant transformation Genomic fragments and reporter constructs were introduced into pBluescript II KS vector (Stratagene, pBlueMLAPUCAP) using Seamless Ligation Cloning Extract (SLICE) (Zhang et al., 2012). ..

    Cloning:

    Article Title: Successive duplication-divergence mechanisms at the RCO locus contributed to leaf shape diversity in the Brassicaceae.
    Article Snippet: .. Molecular cloning and plant transformation Genomic fragments and reporter constructs were introduced into pBluescript II KS vector (Stratagene, pBlueMLAPUCAP) using Seamless Ligation Cloning Extract (SLICE) (Zhang et al., 2012). ..



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